Investigating PIK3R3 and ATp2A1 Genes Expressions in Ventilator-Associated Pneumonia Patients Admitted to the Intensive Care Unit of Masih Daneshvari Hospital in 2016

Abstract

Background: Infectious diseases such as ventilator- associated pneumonia (VAP) are one of the serious problems in intensive care units (ICU) of hospitals. To date, there has been no appropriate clinical and diagnostic marker for early detection of this disease. In this study, expression of PIK₃R₃ and ATp₂A₁ genes in patients with VAP were assessed to be used as biomarkers to identify and confirm the disease. Methods: This study was conducted by using peripheral blood samples of 60 individuals, including 30 patients with VAP and 30 healthy volunteers. First, the peripheral blood samples were taken and then RNA was extracted and converted into cDNA. Finally, the assessment of genes was performed by Real-time PCR. Results: In peripheral blood samples, 46.6% and 30% were positive for PIK₃R₃ expression in patients and healthy groups, respectively. The ATp₂A₁ expression in patients and healthy controls were found 40% and 23.3%, respectively. Comparing the ΔCT obtained for the PIK₃R₃ and ATp₂A₁ genes showed statistically significant differences between the two groups of patients and healthy subjects (p=0.042, p=0.036). Conclusion: ATp₂A₁ and PIK₃R₃ may be used as biomarkers for early detection of VAP disease. However, further studies are required. Key Words: ATp₂A₁ gene, PIK₃R₃ gene, Ventilator- associated pneumonia (VAP)