Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D Printed hybrid scaffolds based on polycaprolactone nanohydroxyapatite/alginate gelatin for bone regeneration

https://cmfrc.tums.ac.ir//Article/Enhancing-in-vitro-osteogenic-differentiation-of-mesenchymal-stem-cells-via-sustained-dexamethasone-delivery-in-3D-Printed-hybrid-scaffolds-based-on-polycaprolactone-nanohydroxyapatite⁄alginate-gelatin-for-bone-regeneration

Abstract Despite the natural ability of bone repair, its limitations have led to advanced organic-inorganic-based biomimetic scaffolds and sustained drug release approaches. Particularly, dexamethasone (DEX), a widely used synthetic glucocorticoid, has been shown to increase the expression of bone-related genes during the osteogenesis process. This study aims to develop a hybrid 3D-printed scaffold for controlled delivery of dexamethasone. Hence, hybrid scaffolds were fabricated using a layer-by