Protein engineering of recombinant human bone morphogenetic protein 2 with higher interaction with Ca phosphate based scaffold used for osteogenesis

https://cmfrc.tums.ac.ir//news/Protein-engineering-of-recombinant-human-bone-morphogenetic-protein-2-with-higher-interaction-with-Ca-phosphate-based-scaffold-used-for-osteogenesis

Abstract The aim of the present study was to assess the recombinant bonemorphogenetic protein 2 (RHBMP-2) with higher substantively and solubility for use in calcium phosphate scaffolds for better release in differentiation of mesenchymal stem cells to osteoblast cells. Using bioinformatics tools, two mutations (p. L10D and p. S12E) were chosen and applied in BMP2 CDS sequence to increase interaction with calcium derived composite. The new recombinant mutated sequence (BMP2mut) was synthesized a