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Optimization of GFP model protein synthesis using Cell free expression system
Summary of the necessity of project implementation
Cell-free expression systems (CFE for short) by using the protein factory of cells, provide the ability to transcribe and produce protein encoded by genes on a DNA molecule in the test tube environment and outside of living cells. Among the advantages of this system compared to classical protein expression systems, there is no need to transform the vector into the cell, use all the available resources to produce the target protein, the possibility of producing toxic proteins for living cells, precise control of the reaction conditions and reducing He mentioned the possibility of Inclusion Body formation. The applications of this system include the study of molecular mechanisms, the production of recombinant proteins, the production of antibodies, the bioproduction of medicinal substances, the development of sensors and the prototyping of genetic circuits.
Moderator: Dr. Mohammad Ali Mazloumi
Start year: 2021
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